PHENOTYPING AND MOLECULAR MARKER ANALYSIS OF WH1105 AND KHARCHIA 65 BACKCROSSES AND F4 PROGENIES OF WHEAT FOR SALINITY TOLERANCE

VARSHA¹*, S. YASHVEER², V. SINGH³, P. VERMA^4
1Department of Molecular Biology, Biotechnology and Bioinformatics, COBS&H, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, Haryana, India
²Department of Molecular Biology, Biotechnology and Bioinformatics, COBS&H, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, Haryana, India
³Department of Genetics and Plant Breeding, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, Haryana, India
⁴Department of Molecular Biology, Biotechnology and Bioinformatics, COBS&H, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, Haryana, India
* Corresponding Author : varshasingh2794@gmail.com

Received : 01-06-2020     Accepted : 27-06-2020     Published : 30-06-2020
Volume : 12     Issue : 12       Pages : 9995 - 10000
Int J Agr Sci 12.12 (2020):9995-10000

Keywords : Nax1, Nax2, Polymorphism, SSRs, PCA, Cluster analysis
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Molecular Biology, Biotechnology and Bioinformatics, COBS&H, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, Haryana, India
Author Contribution : All authors equally contributed

Wheat cultivar, WH1105 is widely grown due to many agronomically superior qualities but is not tolerant to soil salinity. Hence a molecular backcross breeding program was initiated to introgress two salinity tolerant genes (Nax1 and Nax2) from a well-known salinity tolerant cultivar Kharchia65 into WH1105. BC1F3, BC2F2 and F4 generations of the cross WH1105×Kharchia65 were evaluated for various morphological traits under salt-stress condition. On the basis of phenotypic and genotypic variations, 43 high yielding plants were selected from the cross. Out of 178 SSRs tested, 30 were polymorphic for background selection of the foreground selected plants. Cluster analysis of selected plants of all three generations with parents showed that all the selected plants have high similarity with recurrent parent (WH1105). Four plants were selected as high grain yielding and salt tolerant which could be further backcrossed with recurrent parent to develop salt-tolerant wheat lines.

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