BIOSYNTHESIS OF SILVER NANOPARTICLES AND ITS USAGE AGAINST ANTHRACNOSE FUNGUS COLLETOTRICHUM CAPSICI

V.D. CHANDRASHEKHARA¹, S. LOKESH²*, V.V. KAVYASHRI³, M.K. PRASANNA KUMAR⁴, SANGONDDA⁵, M.E. PUNEETH^6
1Department of Studies in Biotechnology, University of Mysore, Manasagangothri, Mysuru, 570006, Karnataka, India
²Department of Studies in Biotechnology, University of Mysore, Manasagangothri, Mysuru, 570006, Karnataka, India
³Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, Bengaluru, 560065, Karnataka, India
⁴Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, Bengaluru, 560065, Karnataka, India
⁵Department of Seed Science & Technology, College of Agriculture, University of Agricultural Sciences, Dharwad, 580005, Karnataka, India
⁶Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, Bengaluru, 560065, Karnataka, India
* Corresponding Author : boramma@rediffmail.com

Received : 02-05-2020     Accepted : 13-05-2020     Published : 15-05-2020
Volume : 12     Issue : 9       Pages : 9843 - 9846
Int J Agr Sci 12.9 (2020):9843-9846

Keywords : Silver, Chilli, Anthracnose, Nano
Academic Editor : Dr Romila Akoijam, Dr R. S. Umakanth
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Institute of Excellence, University of Mysore, Manasagangothri, Mysuru, 570006, Karnataka, India. Authors are also thankful to Department of Studies in Biotechnology, University of Mysore, Manasagangothri, Mysuru, 570006, Karnataka, India
Author Contribution : All authors equally contributed

Green synthesis of nanoparticles is an emerging simple and viable alternative eco- friendly method for chemical and physical methods of plant disease management. In the present study silver nanoparticles have been synthesised using aquatic weed Kappaphycus alvarezii extract and characterized based on XRD and which exhibited maximum absorption at 430 to 440nm which is (SPR) for silver nanoparticles and based on Scanning Electron Microscopy (SEM) nanoparticle size was found to be in the ranges from 60 to 90nm. The XRD analysis of synthesized silver nanoparticles showed four peaks at 38.36, 44.48, 64.62 and 77.62 angles. Also, at 38.36 = 2?, the curve showed the highest peak indicated the crystal shape of nanoparticles. The antifungal activity of the silver nanoparticles checked against Colletotrichum capsici at different concentration i.e., 25, 50, 100, 150, 200, 400 and 600ppm, showed significant zone of inhibition with reduced sporulation and mycelial growth on PDA amended with 200ppm of silver nanoparticles in vitro. This has promised that the newly synthesised silver nanoparticles could be used as an antifungal agent for crop disease management especially in case of anthracnose.

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