HUMORAL RESPONSES TO PLASMODIUM FALCIPARUM BLOOD-STAGE AND PRE-ERYTHROCYTIC ANTIGENS IN INDIVIDUALS LIVING IN TWO SETTINGS WITH DIFFERENT EPIDEMIOLOGICAL STRATA IN COTE D IVOIRE

AT OFFIANAN¹*, SS YAO², NL TIACOH³, SB ASSI⁴, D KOFF⁵, AAB AKO⁶, AA KONE⁷, AE GBESSI⁸, K TUO⁹, S BEOUROU¹⁰, O OUATTARA¹¹, B KONE¹², K LAWSON¹³, J DJAMAN^14
1Malariology Department, Institut Pasteur of Cote D Ivoire
²Malariology Department, Institut Pasteur of Cote D Ivoire
³Malariology Department, Institut Pasteur of Cote D Ivoire
⁴Institut Pierre Richet, Institut National de sante Publique Bouake Cote D Ivoire
⁵Malariology Department, Institut Pasteur of Cote D Ivoire
⁶Malariology Department, Institut Pasteur of Cote D Ivoire
⁷Malariology Department, Institut Pasteur of Cote D Ivoire
⁸Malariology Department, Institut Pasteur of Cote D Ivoire
⁹Malariology Department, Institut Pasteur of Cote D Ivoire
¹⁰Malariology Department, Institut Pasteur of Cote D Ivoire
¹¹Formation Sanitaire Dar Es Salam Bouake Cote D Ivoire
¹²Formation Sanitaire Dar Es Salam Bouake Cote D Ivoire
¹³Centre de Sante Urbain de Libreville, Man Cote D Ivoire
¹⁴Biochemestry Department, Institut Pasteur/UFR Biosciences Felix Houphouet-Boigny University, Abidjan, Cote D Ivoire
* Corresponding Author : andre_offianan@yahoo.fr

Received : 03-08-2018     Accepted : 26-09-2018     Published : 30-10-2018
Volume : 10     Issue : 1       Pages : 205 - 210
Int J Parasitol Res 10.1 (2018):205-210

Keywords : Humeral response, Plasmodium falciparum, Antigens, Cote D Ivoire
Academic Editor : Dr Prasanta Saini, Franck Remoue
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Institut Pasteur of Cote D Ivoire for to financial support. Authors are also thankful to volunteers who agreed to participate in this study, medical staff and all health authorities in collaboration
Author Contribution : All authors participated in the design, implementation, and analysis or data interpretation of the study. OAT was involved in all phases of the study and has full access to all the data. Assi SB, Beourou S and Djaman J Assi SB and Djaman J supervised the

Humoral immune responses play a pivotal role in naturally acquired immunity to malaria. Several Plasmodium falciparum antigens have been described as targets of the host immunity. We assessed antibody responses against a panel of P. falciparum antigens in patients with uncomplicated falciparum malaria in two urban malaria-endemic of Côte d’Ivoire with different epidemiological strata. A cross-sectional analysis of blood samples gathered from participants older than 6 months, in Man and Bouake in Côte d’Ivoire with different transmission strata was performed. Standardized ELISA was used to measure total IgG levels against 5 specific-P. falciparum antigens (CSP, LSA3, SALSA, GLURP, AMA1). A total of 151 sera were analyzed. The combined data from the two sites showed a proportion of responders greater than 50% for all the antigens tested. Proportion of IgG responders was greater than 50% except for SALSA (36.73%) at Man site. At Bouake site proportion of IgG responder was less than 50% with CSP (37.50%) and LSA3 (25%). The lowest proportion was observed in lowest age group. Proportion of IgG responder increased with increasing age at the two sites. Variation in the proportion of responders across the two sites was similar. The levels of IgG against AMA1, LSA3, GLURP and SALSA increased with increasing age at Man site. In contrast only IgG level against AMA1 increased while IgG level against CSP decreased with increasing age at Bouake site. Overall, antibody levels against AMA1, CSP, GLURP and LSA3 were gender-independent. These findings may have significant implications in the design of future malaria vaccines trials

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