BIOEFFICACY OF PODOPHYLLOTOXIN AGAINST Plutella xylostella AND Pieris brassicae

RAKESH KUMAR¹*, URVASHI NEGI², KRISHAN CHANDER SHARMA^3
1Regional Horticultural Research and Training Station, Sharbo, Kinnaur, 172107, Himachal Pradesh, India
²Regional Horticultural Research and Training Station, Sharbo, Kinnaur, 172107, Himachal Pradesh, India
³Regional Horticultural Research and Training Station, Sharbo, Kinnaur, 172107, Himachal Pradesh, India
* Corresponding Author : rakeshd4@gmail.com

Received : 05-07-2018     Accepted : 12-07-2018     Published : 15-07-2018
Volume : 10     Issue : 13       Pages : 6539 - 6542
Int J Agr Sci 10.13 (2018):6539-6542

Keywords : Biological activity, Antifeedant, Cabbage, Cauliflower, Plutella xylostella, Pieris brassicae, Podophyllotoxin, Podophyllum hexandrum
Conflict of Interest : None declared
Acknowledgements/Funding : This work was partially supported by the grant provided by the department of Forests, Government of Punjab (India) to the Regional Centre for National Afforestation and Eco-development Board (Ministry of Environment and Forests, Government of India). Author thankful to Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, 173230, Himachal Pradesh, India
Author Contribution : All author equally contributed

Podophyllotoxin was extracted and purified from the roots of Podophyllum hexandrum Royle. and evaluated for repellent, antifeedant as well as for its toxicity against Plutella xylostella and Pieris brassicae, in a feeding deterrence assay, 93.47 % feeding rejection was observed in the treatments with the 3rd instar larvae of P. xylostella at 1200 ppm whereas at the same concentration, the feeding inhibition in the larvae of P. brassicae was 76.11 %. In a no-choice assay both the insects viz. P. xylostella and P. brassicae, consumed minimum area of 0.94 and 1.71 cm2 after one day of treatment, respectively whereas in the untreated control, the leaf area consumption was 4.16 and 4.00 cm2. The LC50 values of 210.51 ppm and 11.25 ppm was observed for both the insects viz. Plutella xylostella and Pieris brassicae, respectively.

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