IN VITRO REGENERATION THROUGH ORGANOGENESIS AND EMBRYOGENESIS IN POMEGRANATE CULTIVAR BHAGAWA

A.A. CHHATRE¹*, V.B. BHANDE^2
1Department of Agribiotechnology, Lokmangal College of Agribiotechnology, Wadala, Solapur, Mahatma Phule Krishi Vidyapeeth, Rahuri, 413222, India
²Department of Agribiotechnology, Lokmangal College of Agribiotechnology, Wadala, Solapur, Mahatma Phule Krishi Vidyapeeth, Rahuri, 413222, India
* Corresponding Author : chhatre.amit@gmail.com

Received : 26-05-2018     Accepted : 06-06-2018     Published : 15-06-2018
Volume : 10     Issue : 11       Pages : 6206 - 6208
Int J Agr Sci 10.11 (2018):6206-6208

Keywords : Pomegranate, organogenesis¸ embryogenesis, silver nitrate, BAP
Academic Editor : Dr Amol Shinde, Anita Dhobale, Dr Satish Karande
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to the R&D lab facility provided by Lokmangal Organics Research Development (LORDS), Wadala. Author also thankful to Mahatma Phule Krishi Vidyapeeth, Rahuri, 413222, India
Author Contribution : All author equally contributed

Rapid in vitro propagation of pomegranate in Bhagava cultivar using organogenesis and embryogenesis is conducted.MS basal media along with BAP(1.5mg/L.) has given maximum success in shoot induction. Use of Silver nitrate(0.5mg/it.) along with Adenine sulphate (25mg/L) has shown rapid increase in shoot length while use of BAP (1.0mg/L) and kinetin(1.0mg/L) has given maximum number of multiple shoots. In another experiment, embryogenic callus was developed from explants on MS with BAP (0.8mg/L and 1.0mg/L) .The shoots were obtained from callus using BAP(1.0mg/L), Kinetin (2.0mg/L) and NAA(0.1mg/L). Plant growth was rapid in organogenesis while embryogenesis has responded with more number of multiple shoots and rapid plant growth. Both organogenesis and embryogenesis have resulted in satisfactory rooting and hardening percentage of 80-90%. These results indicate feasibility of using embryogenesis or organogenesis for rapid commercial in vitro propagation in pomegranate.

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