ISSN : 0975-3710
EISSN : 0975-9107
G.U. PREMA1*, K.T. RANGASWAMY2
1Department of Plant Pathology, College of Agriculture, Vijaypur, 586101, University of Agricultural Sciences, Dharwad, 580005, Karnataka
2Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore, 560065, Karnataka
* Corresponding Author : prems.gu@gmail.com
Received : 30-01-2018 Accepted : 08-02-2018 Published : 15-02-2018
Volume : 10 Issue : 3 Pages : 5118 - 5122
Int J Agr Sci 10.3 (2018):5118-5122
DOI : http://dx.doi.org/10.9735/0975-3710.10.3.5118-5122
Keywords : Mungbean yellow mosaic virus (MYMV), Coat protein, Detection, Characterization, Phylogenetic analysis, Nucleotide sequence identity, Amino acid identity
Conflict of Interest : None declared
Acknowledgements/Funding : First author thankfully acknowledges Department of Science and Technology (DST), New Delhi for providing financial assistance for research through Inspire Fellowship
Author Contribution : All author equally contributed
Mungbean yellow mosaic disease is a major constraint in production of mungbean in Karnataka. An expected band size of approximately 1000 bp was amplified from MYMV-CP-F/MYMV-CP-R primers, designed specifically for MYMV. Mungbean yellow mosaic virus (MYMV) was characterized by sequencing the coat protein gene. The actual length of MYMV obtained was 889 bp including 115 bp pre-coat proteins at 5’ end, 774 bp core coat protein and 257 deduced amino acids. The phylogenetic analysis of the coat protein sequence of yellow mosaic virus infecting mungbean was carried out together with the known begomoviruses sequences obtained from Genbank database. Cluster phylogram based comparison of the coding nucleotide sequences of MYMV-Hebbal-Bangalore isolate revealed that the MYMV-Hebbal, Bangalore isolate group with cluster II consisting of Mungbean yellow mosaic viruses. Analysis of nucleotide sequence of coat protein gene of yellow mosaic virus associated with mungbean showed maximum identity with 99% MYMV-Maharashtra: SB [AF314530.1], followed by 98.9% with MYMV-Namakka l: MoB [DQ865201.1] as well as MYMV-Tamil Nadu:MB [AJ132575.1] and 98.8% with MYMV-Madurai: SB isolates [AJ421642.1]. The nucleotide identity of MYMV-Hebbal isolate with other MYMV isolates ranged between 94.5-99%. MYMV-Hebbal, Bangalore isolate showed an identity of 79.3-81.0% with different MYMIV isolates. The amino acid sequence of mungbean yellow mosaic virus shared maximum identity of about 100% with MYMV-Maharashtra: SB [AF314530.1], MYMV-Tamil Nadu:MB [AJ132575.1], 99.6% with MYMV-Namakkal:MoB [DQ865201.1], 99.2% with MYMV-Madurai: SB [AJ421642.1]. The deduced amino acid identity of MYMV-Hebbal-Bangalore isolate with other MYMV isolates ranged between 96.4-100%. The aminoacid identity of MYMV-Hebbal-Bangalore isolate with other MYMIV isolates ranged from 84.0-85.9%.The results from the present study revealed that yellow mosaic virus infecting mungbean from Hebbal, Bangalore is a Mungbean yellow mosaic virus (MYMV) but not Mungbean yellow mosaic India virus(MYMIV) and is a variant of Mungbean yellow mosaic virus(MYMV).
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