COMPARATIVE EFFICACY OF MELATONIN AND TREHALOSE ADDITIVES ON ENZYME LEAKAGE DURING CRYOPRESERVATION OF KANKREJ BULL SEMEN

H.K. THUMAR¹*, M.R. PATEL², H.C. NAKHASHI³, B.N. SUTHAR⁴, T.V. SUTARIA⁵, P.M. CHAUHAN⁶, S.P. DESAI^7
1Department of Veterinary Gynaecology and Obstetrics, Dr. V.M. Jhala Clinical complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
²Department of Veterinary Gynaecology and Obstetrics, Dr. V.M. Jhala Clinical complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
³Department of Veterinary Gynaecology and Obstetrics, Dr. V.M. Jhala Clinical complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
⁴Department of Veterinary Gynaecology and Obstetrics, Dr. V.M. Jhala Clinical complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
⁵Department of Veterinary Gynaecology and Obstetrics, Dr. V.M. Jhala Clinical complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
⁶Department of Veterinary Gynaecology and Obstetrics, Dr. V.M. Jhala Clinical complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
⁷Department of Veterinary Gynaecology and Obstetrics, Dr. V.M. Jhala Clinical complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
* Corresponding Author : thumarharibhai@gmail.com

Received : 06-10-2017     Accepted : 12-12-2017     Published : 30-12-2017
Volume : 9     Issue : 55       Pages : 4940 - 4942
Int J Agr Sci 9.55 (2017):4940-4942

Keywords : Kankrej bull, Cryopreservation, Additives, AST and ALT
Academic Editor : Dr Brijesh Kumar, Prajapati Mayurkumar Manibhai
Conflict of Interest : None declared
Acknowledgements/Funding : This study was supported by Livestock research station, S. K. Nagar and department of veterinary gynaecology and obstetrics, Dr. V. M. Jhala clinical complex Deesa, Sardarkrushinagar Dantiwada Agricultural University, Gujrat, India
Author Contribution : Guidance provided by Dr. H.C. Nakhashi and Dr. B.N. Suthar and other author help in research work and data analysis

Aim of this study to evaluate the comparative efficacy of melatonin and trehalose additives in TFYG extender during cryopreservation of Kankrej bull semen. Total three Kankrej bulls aged between 5 to 6 years were used and semen was collected using artificial vagina method once weekly for eight weeks from each of the bulls. The semen was evaluated for enzymatic leakage at post-dilution, post-equilibration and post-thaw stages of cryopreservation using 2mM Melatonin and 100mM Trehalose concentrations and control without additive. Mean AST and ALT activities were 63.24±1.06 U/L and 22.97±0.71 U/L in 100mM Trehalose group at post-thaw stage, respectively and were significantly (P < 0.05) lower as compared to that of the other groups. Hence, the 100mM Trehalose in TFYG extender for cryopreservation is comparatively better additive in view to preserve semen quality in Kankrej bull.

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