F GENE SEQUENCING AND ANALYSIS OF INDIAN NEW CASTLE DISEASE VIRUS

P.D. RABARI¹*, M.K. JHALA², B.B. BHANDERI³, R.A. MATHAKIYA^4
1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
²Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
³Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
⁴Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
* Corresponding Author : panna.han@gmail.com

Received : 10-02-2017     Accepted : 25-03-2017     Published : 12-04-2017
Volume : 9     Issue : 17       Pages : 4156 - 4158
Int J Agr Sci 9.17 (2017):4156-4158

Keywords : Newcastle Disease, Newcastle Disease Virus, RT-PCR, F gene and commercial poultry farming
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are highly thankful to the Dean, College of Veterinary science and A.H. Anand for financial assistance and research facilities to conduct this research work
Author Contribution : all author equally contributed

Newcastle disease (ND) is one of the most important infectious diseases of poultry. Incidences of ND in vaccinated chicken population of Anand area of Gujarat demanded a study for pathotyping and genetic characterization of the field NDV isolates. Ten allantoic fluid samples from the SPF eggs inoculated with NDV suspected tissue suspension were used for the RNA isolation, followed by one step RT-PCR. All the ten isolates yielded expected 362bp product of F gene indicating them positive for NDV. Multiple sequence alignment of Anand isolates with reference Indian Bareilly strain and two vaccine strains R2B and LaSota revealed 7 unique mutations in Anand isolates. The amino acid sequences of all the ten Anand isolates showed RRQKRF between 112 and 117 positions indicating them to be of virulent type. NDV isolates showed maximum genetic similarity 94.32% with genotype XIII strain (Chicken/Sweden/97) indicating isolates to be of genotype XIII.

1. Aldous E.W. and Alexander D.J. (2001) Avian Pathol., 30, 117-128.
2. Alexander D.J. (2000) Avian.Pathol., 29, 95-100.
3. Alexander D.J. and Enne D.A. (2008) Newcastle Disease and Other Avian Paramyxoviruses. In: A Laboratory Manual for the Isolation, Identification and Characterization of Avian Pathogens, Dufour-Zavala L. (Editor in Chief) Swayne, D. E., Glisson, J. R., Jackwood, M. W., Pearson, J. E., Reed, W. M, Woolcock, P. R., 4th ed., American Association of Avian Pathologists, Athens, pp. 135–141.
4. Boostani A.R., Pourbakhsh S.A., Momayez R. and Charkhkar S. (2012) African Journal of Microbiology Research, 7(8), 657-660.
5. Chenna R., Sugawara H., Koike T., Lopez R., Gibson T.J., Higgins D.G. and Thompson J.D. (2003) Nucleic Acids Res., 31, 3497-3500.
6. Collins M.S., Bashiruddin J.B. and Alexander D.J. (1993) Archives of virology., 128, 363-370.
7. Kant A.G., Koch F. Roozelaar, Balk F., Ter Huurne A. (1997) Avian Pathol., 26, 837–849.
8. Kumar A., Maan S., Mahajan N.K., Rana V.P., Jindal N., Batra K., Ghosh A., Mishra V.P., Kapoor S. and Maan N.S. (2013). Indian J. Virol., 24(3), 380-385.
9. Nwankiti O.O., Ejekwolu A.J., Ibrahim I., Ndako J.A. and Echeonwu G.O.N. (2010) Detection of Serum Antibody levels against Newcastle Disease In local chickens in Bauchmetropolis, Bauchi state, Nigeria.
10. OIE (2012) New Castle Disease In: Manual of Diagnostic Tests and vaccines for Terrestrial Animals Paris: Office International des Epizootics, pp
11. Pedersen J.C., Senne D.A., Woolcook P.R., Kinde H., King D.J., Wise M.G., Panigraby B. and Seal B.S. (2004) J. Clin.Microbiol., 42, 2329-2334.
12. Susta L., Jones M.E.B., Cattoli G., Cardenas-Garcia S. , Miller P.J., Brown C.C., Afonso C.L. (2014) Veterinary Pathology, Vol 52, Issue 1, pp. 120 - 131.
13. Tamura K., Stecher G., Peterson D., Filipski A. and Kumar S. (2013) Molecular Biology and Evolution, 30, 2725-2729.