EVALUATION OF MOLECULAR BIOLOGY FOR IDENTIFICATION THEILERIOSIS DISEASE IN CROSSBRED CATTLE

A.P. PADHIYAR¹, K.M. JADHAV², BHARATKUMAR K. PATEL³*, H.C. CHAUHAN⁴, KIRIT B. PATEL^5
1Department of Medicine and Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Deesa, 385535, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat 385506
²Department of Medicine and Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Deesa, 385535, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat 385506
³Department of Medicine and Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Deesa, 385535, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat 385506
⁴Department of Medicine and Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Deesa, 385535, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat 385506
⁵Department of Medicine and Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Deesa, 385535, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat 385506
* Corresponding Author : bharatpatel1063@yahoo.com

Received : 03-10-2016     Accepted : 25-12-2016     Published : 28-12-2016
Volume : 8     Issue : 63       Pages : 3575 - 3577
Int J Agr Sci 8.63 (2016):3575-3577

Keywords : Screening for sample, Microscopy observation, PCR, Drug efficacy
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are thankful to the Department of Medicine and Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, S.K. Nagar for providing the facilities for this work
Author Contribution : All authors are associated with this works like sample collection, guide, technical support, literature collection and manuscript writing respectively

Theileriosis is hemoprotozoan disease spread in most of developing countries including India. In this presence study was done with cattle of north Gujarat India 44 and 40 blood sample were collected from crossbred cattle respectively clinical case and subclinical case out of 282 crossbred cattle. Only 13 sample was conform Theileriosis positive detected in microscopic examination in group of clinical case but not in sub clinical case but the detection done by polymerase chain reaction (PCR) were showed 30 and 21 sample positive respectively in clinical case and subclinical case. In other word 15.4% and 60.7% sample showed positive respectively microscopic and molecular technique. It means the PCR was fine technique for detection of Theileriosis. The different group of cattle was treated with different antibiotic combination, Buparvaquone was proved effective than other drug.