MOLECULAR PHYLOGENY OF VENERIDAE (MOLLUSCA:BIVALVIA) BASED ON NUCLEAR RIBOSOMAL INTERNAL TRANSCRIBED SPACER REGION

AMPILI M.¹*, SREEDHAR S.K.^2
1Department of Zoology, N.S.S. Hindu College, Changanassery- 686 102, Kerala, India.
²Department of Zoology, S.N. College, Cherthala- 688 530, Kerala, India.
* Corresponding Author : ampilirajeev@gmail.com

Received : 03-04-2014     Accepted : 24-04-2014     Published : 05-05-2014
Volume : 5     Issue : 1       Pages : 96 - 101
Int J Mol Biol 5.1 (2014):96-101
DOI : http://dx.doi.org/10.9735/0976-0482.5.1.96-101

Conflict of Interest : None declared

In the present study, molecular phylogeny of bivalve family Veneridae (Mollusca:Bivalvia) was analysed using internal transcribed spacer (ITS) region of 21 species belonging to different subfamilies of Veneridae. ITS of ribosomal DNA can be utilised for delineating evolutionary and genetic relationships between closely related taxa. ITS region of Paphia malabarica belonging to subfamily Tapetinae and Meretrix casta belonging to meretricinae was sequenced. Total genomic DNA was extracted from the adductor muscle using CTAB protocol and the internal transcribed spacer region of nuclear ribosomal DNA was PCR amplified and sequenced using ITS (ITS1 and ITS2) forward and reverse primers. Total length of sequence was found to be 895 bp in Paphia malabarica and 785 bp in Meretrix casta. GC contents in the sequences were found to be 58.99% and 64.68% respectively in Paphia malabarica and Meretrix casta. ITS1 region of Paphia malabarica consisted of 393 bp with GC content 58.12% and 309 bp with 63.75% GC content in Meretrix casta. ITS2 region generated 248bp with 63% GC content in Paphia malabarica and 240bp with 72.06% GC content in Meretrix casta. 5.8S region rendered 157bp with 60% GC content in Paphia malabarica and 138bp with 61.32% GC content in Meretrix casta. Phylogenetic trees were constructed using Neighbor-Joining and Maximum-Parsimony methods. The analysis produced trees with similar topology. The tree topologies indicated two clades with three well resolved groups. Phylogenetic analysis supported the morphological classification and monophyly of the family Veneridae.

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