MORPHOLOGICAL AND MOLECULAR ASSESSMENT OF GENETIC DIVERSITY OF WATER CHESTNUT (Trapa natans) FROM DIFFERENT PONDS OF CENTRAL INDIA

KACHARE S.¹, TIWARI S.², TRIPATHI N.^3
1Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur- 482 004, MP, India.
²Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur- 482 004, MP, India.
³Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur- 482 004, MP, India.

Received : 12-11-2013     Accepted : 12-12-2013     Published : 31-12-2013
Volume : 1     Issue : 2       Pages : 28 - 31
World Res J Cell Biol 1.2 (2013):28-31

Twenty Random Amplified Polymorphic DNA markers were used to investigate genetic diversity among 18 genotypes of Trapa natans. A total of 93 bands were detected, among which 50 bands (53.76%) were polymorphic. The average number of band per primer was 4.8, while average number of polymorphic band was 2.6. The sizes of amplified products were ranged from 150-2000bp. Low levels of average genetic diversity was observed. A dendrogram produced by the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) based on Jaccard’s similarity coefficient revealed two groups. These findings could assist in defining the best method of genetic conservation and studies in breeding programs for genetic improvement of T. natans.