DETERMINATION OF PHYSICAL EXISTENCE OF BACTERIAL LIPASE CODING GENE FOR STABLE EXPRESSION

RATHORE M.¹, GAJULA P.², SHARMA K.³, SHARMA N.^4
1Microbial Research Laboratory, UCoS, MLS University, Udaipur- 313 001, Rajasthan, India.
²Indian Agricultural Statistics Research Institute, Pusa Campus, New Delhi-110012, Delhi, India.
³Microbial Research Laboratory, UCoS, MLS University, Udaipur- 313 001, Rajasthan, India.
⁴Indian Agricultural Statistics Research Institute, Pusa Campus, New Delhi-110012, Delhi, India.

Received : 05-11-2013     Accepted : 03-12-2013     Published : 31-12-2013
Volume : 1     Issue : 2       Pages : 19 - 21
World Res J Cell Biol 1.2 (2013):19-21

Industrial as well as medical application of bacterial enzyme requires the stable expression of enzyme coding gene. As any physical and chemical shock might lead to removal of plasmid hence genome borne characteristics are more stably inherited and expressed rather than plasmid borne. In order to determine existence of lipase coding gene, plasmid curing experiments were performed on twelve lipolytic bacteria isolated from food samples. Ethidium bromide was used as a most suitable bacterial plasmid curing agent. Results suggested that cured strains retained the characteristics of lipolysis and which in turn suggest that it is genome borne characteristic in all strains. Since genome stably inherited from one generation to another hence these liploytic strains can be very useful for industrial as well as medical applications. Further sequencing and cloning experiments will be very helpful to develop high expression system for this lipase coding gene. This study serves as a guide to monitor the basic steps in the identification of the genes responsible for lipase production; provides a basis to identify a target site for future genetic manipulation such as cloning, amplification, deletion and transfer of genes require for isolation of stable and high expression of lipase production from bacterial isolates.