FLAP ENDONUCLEASE 1(FEN1) INTERACTION STUDY WITH PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) THROUGH HOMOLOGY MODEL AND DOCKING

BISWAS S.¹, KUNDU S.^2
1Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, Kolkata-700 009, WB, India.
²Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, Kolkata-700 009, WB, India.

Received : 13-11-2013     Accepted : 09-12-2013     Published : 27-12-2013
Volume : 1     Issue : 1       Pages : 12 - 18
World Res J Bioinformatics 1.1 (2013):12-18

Conflict of Interest : None declared

PCNA and Fen1 proteins play a very crucial role within cell to complete replication process and other cell cycle activities. Absence of experimentally solved protein structures of PCNA and Fen1 of Methanosarcina mazei in archaeal domain of life inspire us to build homology models of these proteins to understand the interactions between these very important cell cycle proteins at the residue level. M.mazei is one of the rare archaeal organism that inhabit in diverse environmental conditions, having both prokaryotic and eukaryotic nature and also has the ability to produce methane. Interactions natures of these two core replication processing proteins in such a interesting organism were studied by docking of homology models, electrostatic surface potential of individual proteins and their complexes. Although evolutionarily human is more evolved organism but this study confirm PCNA and Fen1 interact similarly in M.mazei through a PCNA inter domain connecting loop and PIP-box motif of Fen1 protein. However, actual residues involved between these two proteins in two different domain of life have evolved and mutate over the course of evolution. So, from the present study we can conclude that the core replication processing proteins like PCNA and Fen1 interact in similar fashion whether it’s a higher eukaryote like human or methane producing archaeal organism like M. mazei.