ELECTRON BEAM IRRADIATION OF GLASS IONOMER CEMENT TYPE IX ON HUMAN ERYTHROCYTES- A CYTOTOXIC EVALUATION

HEGDE M.N.¹, SHABIN S.², HEGDE N.D.³, SUCHETHA K.⁴, SANJEEV G.⁵, SHETTY S.^6
1A.B. Shetty Memorial Institute of Dental Sciences- 575 018, Nitte University, Mangalore, Karnataka, India.
²A.B. Shetty Memorial Institute of Dental Sciences- 575 018, Nitte University, Mangalore, Karnataka, India.
³A.B. Shetty Memorial Institute of Dental Sciences- 575 018, Nitte University, Mangalore, Karnataka, India.
⁴K.S. Hegde Medical Academy, Deralakatte, Mangalore- 575 018, Karnataka, India.
⁵Microtron Centre, Department of Physics, Mangalore University, Mangalore- 574 199, Karnataka, India.
⁶A.B. Shetty Memorial Institute of Dental Sciences- 575 018, Nitte University, Mangalore, Karnataka, India.

Received : 13-11-2013     Accepted : 09-12-2013     Published : 26-12-2013
Volume : 3     Issue : 1       Pages : 34 - 36
Adv Med Informat 3.1 (2013):34-36

Aim: The aim of this study was to evaluate the cytotoxic potential of glass ionomer cement type IX available before and after electron beam irradiation. Materials and Methods: After blood collection, erythrocyte was separated and kept for cytotoxic study. A total of 20 samples of GC Fugi IX were prepared on rectangular bar shaped specimens of 25-× 2-× 2- mm and samples were divided into two categories: Non radiated and Radiated. 10 samples of GC fugi IX were irradiated at 10 KGy (Microtrone Centre, Mangalore University, India). The cement extraction was done for a duration of 24 hours, 7 days and 14 days. The test solutions were sterile filtered using a Sterile Filter Unit (0.2 μm pore size) (Sartorius Stedim, Biotech, Germany) and was subjected to the cytotoxicity assay. Results: Non radiated samples of Fugi type IX on human erythrocytes showed that the percentage of hemolysis was more in non radiated samples than radiated samples after 24 hours and 14 days. The percentage of hemolysis was reduced in radiated samples after 7 days and 14 days. Conclusions: The reduction in hemolysis after electron beam irradiation of samples may be due to cross linking of unbound acids and increase in hemolysis of non radiated samples may be due to the presence of unbound acids.