MINERAL STATUS OF ANIMALS IN RELATION TO DIFFERENT PHYSIOLOGICAL STAGES IN HARIDWAR DISTRICT OF UTTARAKHAND

Tiwary M.K.¹, Akhilesh Pandey², Tiwari D.P.^3
1Touring Veterinary Officer, Bengabad, Giridih (Jharkhand)
²Department of Animal Genetics and Breeding, C.O Vs. Jabalpur, India
³Department of Animal Nutrition, C.V. A. Sc., G.B. P.U.A. & T., Pantnagar, UA, India

Received : -     Accepted : -     Published : 15-06-2010
Volume : 1     Issue : 1       Pages : 1 - 9
Food Sci Tech Lett 1.1 (2010):1-9

Conflict of Interest : None declared
Acknowledgements/Funding : Financial help provided by AICRP, New Delhi to carry out this study is duly acknowledged.

An experiment was conducted in four villges from two tehsils, namely Roorkee and Laksar of Haridwar district of Uttarakhand. Two villages from each tehsil were indentified from where minimum 10-15 farmers of different categores in each village were selected. Blood samples from cattle and buffaloes of different physiological status viz., lactating, dry, heifer, pregnant and other reproductive health were collected from jugular vein in sterilized and vacuumized test tube. During analysis the serum samples with equal volume of concentrated HNO3 were kept for overnight in a digestion tube followed by low heat (70-800C) digestion with diacid mixture. The final content was filtered through Whatman’s filter paper No. 1 (Kolmer et al., 1951). Minerals such as Ca, Mg, Zn, Fe, Cu and Mn were estimated by using AAS (Atomic Absorption Spectrophotometry). Phosphorus in blood serum (Fiske and Subbarow, 1925) using Autozyme Phosphorus kit, (ACCUREX Biomedical Pvt. Ltd.) was estimated colorimetrically. The overall blood serum mineral concentration for Ca (10.43±022 mg/dl), Mg (1.65±0.14 mg/dl), Zn (1.54±0.11 ppm), Fe (1.97±0.19 ppm) and Mn (0.54±0.02 ppm) in cattle and buffaloes of different physiological status were found above their respective critical levels whereas, Ps (3.79 ± 0.21 mg/dl) and Cu (0.46 ± 0.07 ppm) concentrations in blood serum of animals were below the respective critical levels. The average serum Ca, Mg, Zn and Fe differ significantly (P<0.05) between the Roorkee and Laksar tehsils of Haridwar district of Uttarakhand. There was significantly (P<0.05) higher serum Ca, Mg, Zn and Fe levels in animals of Laksar tehsil (10.82±0.31 mg/dl, 1.88±0.16 mg/dl, 1.92±0.14 ppm and 2.44±0.29 ppm, respectively) than in animals of Roorkee tehsil (10.03±0.25 mg/dl, 1.42±0.22 mg/dl, 1.16±0.06 ppm and 1.49±0.14 ppm, respectively). Most of the animals were found to have reproductive problems, which could be attributed to deficiency of different important minerals in this region. To overcome the deficiency, strategic dietary supplementation of minerals with better bioavailability could be a suitable approach.

[1] Biswas R. and Samanta G. (2002) Indian J. Anim. Sci., 72 (1): 104- 106  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[2] Chew B.P. (2000) Micronutrients play role in stress, production in dairy cattle  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[3] Das A., Biswas P. and Rajendran D. (2003a) Anim. Nutr. Feed Tech., 3: 9 –16  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[4] Das A., Ghosh T. K. and Haldar S. (2003b) Indian J. Anim. Sci., 73 (4): 448-454  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[5] Das P., Biswas, S., Ghosh T. K. and Haldar S. (2002) Anim. Nutr. and Feed Tech., 2: 19-26  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[6] Fiske C. H. and Subbarow Y. (1925) J. Biolog. Chem.., 66: 375-378.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[7] Gowda N.K.S., Prasad C.S., Ramana, J.V. and Shivaramaiah M.T. (2001) Anim. Nutr. Feed Tech., 1 (2): 97–104  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[8] Kaneko J.J., Harvey J. W. and Bruss M.Z. (1997) In: “Clinical Biochemistry of Domestic Animals”. 5th edn. Academic Press, USA  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[9] Kolmer J.A., Spanbling E.H. and Robinson H.W. (1951) Approved laboratory techniques. Appleton Century Crafts, New York.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[10] Kumar M. (1993) Availability of various nutrients in different varieties of sorghum exaluated by nylon bag technique. Thesis M.V.Sc. G.B.P.U.A&T. Pantnagar  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[11] McDowell L.R. (1985) Nutrition of grazing ruminants in warm climates. Academic press. New York  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[12] Miles, W.H. and McDowell, L.R. (1983). Mineral deficiency in the Llanos ranges. World Animal Review, 46: 2-10  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[13] Ramana J.V., Prasad C.S., Gowda N.K.S. and Ramchandra K.S. (2001) Indian J. Anim. Nutr., 18 (3): 235-242  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[14] Samanta A. and Samanta G. (2002) Indian J. Anim. Nutr., 19 (3): 278-281.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[15] Sharma M.C. and Joshi C. (2004) Indian J. Anim. Sci., 74 (7): 775- 779  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[16] Sharma M.C., Joshi C. and Sarkar T.K. (2002) Asian-Aust. J. Anim. Sci. 15 (9): 1278-1287.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[17] Sharma M.C., Joshi C. and Sarkar T.K. (2003) Indian. J. Anim. Sci., 73 (3): 308-311  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[18] Snedecor G.W. and Cochran W.G. (1994) Statistical Methods. Iowa State University press Ames, IOWA, USA.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[19] Tandle M.K., Amanullah M., Honnappagol S.S., Kartikesh S.M., Ram J. and Sonwane S.D. (1997) Indian J. of Agricultural Research. 18 (1): 44-45  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[20] Underwood E.J. (1981) The Mineral Nutrition of Livestock. Common Wealth Agriculture Bureux, London, England  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[21] Underwood E.J. and Suttle N.F. (1999) The mineral nutrition of livestock (3rd edition). CABI Publishing, CAB International, Wallingford, U.K  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[22] Vanaken D., Bont J.D., Van Holm L. and Ranawana S.S.E. (1991) Ind. Vet. J.,3 (6): 371-374.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[23] Yadav P.S., Mandal A.B., Kapoor V.K., Sunaria K.R., and Maan N.S. (1998) Indian J. Anim. Sci. 68: 1059-1061  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[24] Part of M.V.Sc. Thesis submitted by the first author to G. B. P. U. A. & T., Pantnagar  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus