THE ADAPTIVE CHANGES OF THE OXIDATIVE PENTOSE PHOSPHATE PATHWAY IN RESPONSE TO OXIDATIVE STRESS INDUCED BY COPPER AND ZINC DEFICIENCY IN LUNG OF FEMALE RATS

FARES K KHALIFA¹*
¹Biochemistry and Nutrition Department, Women’s College, Ain Shams University, Cairo, Egypt.
* Corresponding Author : dr_fares_asu@yahoo.com

Received : 21-12-2011     Accepted : 29-12-2011     Published : 30-12-2011
Volume : 2     Issue : 1       Pages : 10 - 15
J Clin Res Lett 2.1 (2011):10-15
DOI : http://dx.doi.org/10.9735/0976-7061.2.1.10-15

Keywords : Oxidative stress, Pentose phosphate pathway, Copper-Zinc deficiency
Conflict of Interest : None declared

The purpose of the present study was to assess and examine the links among zinc and/or copper deficiency, oxidative stress and the adaptive changes of the oxidative phase of the pentose phosphate pathway in lung of adult female rats fed diets deficient in copper and/or zinc. Four groups each of ten adult female Albino rats (Sprague-Dawely) strain, mean weight varied between 80.2 to 82.5 g were fed on basal control diet (CD), copper deficient diet (-CuD), zinc deficient diet (-ZnD), and diet deficient in both of copper and zinc (-CuZnD). Serum and lung were analyzed for enzymatic and nonenzymatic primary defense system components. Serum and lung Cu and Zn levels were lowered significantly (p<0.01) in – CuD, -ZnD, and -CuZnD rats. The activities of erythrocyte CuZnSOD, lung catalase and GPx were reduced in all deficient rats. Lung content of GSH, GSSG, and ascorbate were significantly reduced by feeding Cu and/or zinc deficient diets. Serum MDA content was increased in rats fed diets deficient in copper and/or zinc groups. Lung activities of oxidative pentose phosphate pathway enzymes (G6PDH and 6PGDH) were significantly increased by Cu and/or zinc deficiency. The present study concluded that, the oxidative stress induced by feeding diets deficient in Cu and/or Zn result in changes in the oxPPP enzymes activities (G6PDH and 6PGDH). The oxPPP maintains the redox potential and adapts these changes to protect against oxidative stress by NADPH generation, which is required for detoxification of free radicals and peroxides.

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