Effect of temperature on cellulose enzyme activity in crude extracts isolated from solid wastes microbes

Nataraja S.¹, Chetan D.M.², Krishnappa M.^3
1Department of Botany Sahyadri Science College, Kuvempu University-Shimoga, 577202
²Department of Biotechnology NMAM Institute of Technology- Nitte, 574110
³Professor Department of studies and research in Applied Botany Kuvempu university Shankaraghatta, 577 451

Received : -     Accepted : -     Published : 21-12-2010
Volume : 2     Issue : 2       Pages : 44 - 47
Int J Microbiol Res 2.2 (2010):44-47
DOI : http://dx.doi.org/10.9735/0975-5276.2.2.44-47

Keywords : Cellulose, Cellobiohydrolase, hydrolases
Conflict of Interest : None declared

Cellulose is degraded by three major classes of hydrolases. These three classes have been isolated and purified from fungi. They range widely in temperature and pH optima, but the most common enzymes are most active at pH 5.5, 55oC.Three cellulases are of interest: 1) endoglucanase (carboxymethyl cellulase or CMCase), 2) cellobiohydrolase (CBH or filter paper activity) and 3) β- glucosidase. Endoglucanase (CMCase) attacks randomly in the interior of the cellulose structure. It is not very active against crystalline cellulose, but they are capable of hydrolyzing substituted cellulose such as carboxymethyl cellulose. It produces cellulodextrins also known as cellulooligosaccharides. Cellobiohydrolase (CBH) also known as exocellulase attacks crystalline cellulose from the non-reducing end and produces cellobiose. β-glucosidase hydrolyses cellobiose to glucose.

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