Optimization of production and partial purification of laccase by Phanerochaete chrysosporium using submerged fermenation

Jhadav A.¹, Vamsi K.K.², Khairnar Y.³, Boraste A.⁴, Gupta N.⁵, Trivedi S.⁶, Patil P.⁷, Gupta G.⁸, Gupta M.⁹, Mujapara A.K.¹⁰*, Joshi B.¹¹, Mishra D.^12
1Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
²Rai foundations College CBD Belapur Navi Mumbai
³Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
⁴S.V.P.M. College of Pharmacy, Baramati, Pune
⁵Sindhu Mahavidyalaya Panchpaoli Nagpur
⁶V.V.P. Engineering College, Rajkot, Gujrat
⁷Dr. D. Y. Patil ACS College, Pimpri, Pune
⁸S.D.S.M. College Palghar, Mumbai
⁹Sindhu Mahavidyalaya Panchpaoli Nagpur
¹⁰Sir PP Institute of Science, Bhavnagar, Gujrat
¹¹Rural College of Pharmacy, D.S Road, Bevanahalli, Banglore
¹²Senior Research Coordinator, Reliable analytical laboratory, Thane
* Corresponding Author : adarshmujapara@gmail.com

Received : -     Accepted : -     Published : 21-12-2009
Volume : 1     Issue : 2       Pages : 9 - 12
Int J Microbiol Res 1.2 (2009):9-12
DOI : http://dx.doi.org/10.9735/0975-5276.1.2.9-12

Conflict of Interest : None declared

Laccases are multi copper oxidases having wide substrate specificity mainly found in white-rot fungi, which are the only microorganisms able to degrade the whole wood components. In contrast to most enzymes, which are generally very substrate specific, laccases act on a surprisingly broad range of substrates, including diphenols, polyphenols, different substituted phenols, diamines, aromatic amines, benzenethiols and even some inorganic compounds such as iodine. As they are capable of degrading a wide variety of compounds they are commercially very significant. This project aims at studying the production optimization of laccase using different carbon sources.

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