Optimization of some physical parameters for the production of gluconic acid by a mutant gluconobacter oxydans GPM 60

Ganguly S.¹*, Patra S.K.², Mandal S.K.^3
1Department of Biological Sciences, Sankrail A. C. High School (H.S.), Sankrail, Howrah, West Bengal, India
²Department of Physiology Surendranath College, Kolkata, West Bengal, India
³Department of Organon of Medicine, Midnapore Homeopathic medical college and Hospital, Midnapore, West Bengal, India
* Corresponding Author : subhadeepgangulyphysiol@rediffmail.com

Received : -     Accepted : -     Published : 21-12-2010
Volume : 2     Issue : 2       Pages : 1 - 4
Int J Biotechnol Appl 2.2 (2010):1-4
DOI : http://dx.doi.org/10.9735/0975-2943.2.2.1-4

Keywords : Gluconic acid, mutant, optimization, physical parameters, inoculum
Conflict of Interest : None declared

The microbial production of gluconic acid has been investigated using a mutant Gluconobactor oxydans GPM 60 through optimization of some Physical parameters. Production conditions like initial pH, incubation period, volume of media, age of inoculum, volume of inoculum, agitation and temperature were individually opitimized one by one. The maximum yield of gluconic acid is 13.80 g/L with pH 6.0, 96h incubation, 20 ml medium, 48h age of inoculum, 10 ml volume of inoculum, 250 rpm agitation and 30°C temperature.

[1] Moyer A. J. (1940) US patent No. 2351500, chem. Abstr. 38, 5360  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[2] Rohr M., Kubicek C. P., Kominek J. (1983) Biotechnology, 3  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[3] Rehm H. J., Reed G. (Eds.), Verlag Chemie, Weinheim, Germany (1983) 455 – 465  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[4] Currie J. N., Carter R. H. (1930) US Patent No. 1896811  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[5] Singh O. V., Jain R. K., Singh R. P. (2003) J. Chem. Technol. Biotechnol, 78, 208 – 212  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[6] Moksia J., Larroche C., Gros J. B. (1996) Biotechnol. Lett. 18, 1025 – 1030  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[7] Vassilev N. B., Vassileva M. C., Spassova D. I. (1993) Appl. Microbiol. Biotechnol. 39, 285 – 288  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[8] Stubbs J. J., Lockwood L. B., Roe E. T., Tabenkin B., ward G. E. (1940) Ind. Eng. Chem. 32, 1626 – 1630.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[9] Curie J. N., Kane J. H., Finlay A (1931) US patent No. 1893819. Chem. Abstr 27, 2249. Chem. Abstr 27, 2249  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[10] Everaerts F. M., Beckers J. L., Verheggen T. P. E. M. (1976) Isotachopho. resis – Theory, Instrumentation and Applicatioons, Elsevier, Amsterdam, The Netherlands  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[11] Miller G. L. (1959) Anal. Chem. 31, 426 – 428.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[12] Shah A. H. (1998) PhD Thesis, Quaid – I. Azam University, Islamabad, Pakistan  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[13] Ambati P., Ayyanna C. (2001) World J Microbiol Biotechnol. 17, 331 – 335.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[14] Tran C. T., Mitchell D. A. (1995) Biotechnol lett, 17, 1107 – 1110.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[15] Milsom P. E. (1987) Food Biotechnology – 1, edited by king R. D. and cheetam P. S. J. (Applied Science, London and New York), 273 – 306.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[16] Sankpal N. V., Kulkarni B. D. (2002) Process Biochem. 37, 1343 – 1350.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[17] Nelson R. P. (1974) US. Patent No. 39577580  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[18] Moyer A. J., Welss P.A., Stubbs J. J., Herrick H. T., May O. E. (1937) Ind. Eng. Chem. 29, 777 – 781  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[19] Bernhauer K. (1924) Biochem. Z. 153, 517– 521  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[20] Nakayama K., Kitadah S., Seto Z., Kinoshita S. (1961) J. Gen. Appl. Microbiol. 7, 41 – 51  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[21] Znad H., Markos J., Bales V. (2004) Process Biochem. 39, 1341 – 1345  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[22] Wells P. A., Moyer A. J., Stubbs J. J., Herrick H. T., May O. E., (1937) Ind. Eng. Chem. 29, 635 – 656  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[23] May O. E., Herrick H. T., Moyer A. J., Wells P.A. (1934) Ind. Eng. Chem., 26, 575 – 578  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[24] Stadler – Szoke A., Nyeste L., Hollo J. (1980) Acta Aliment. 9, 155 – 172  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[25] Velizarov S., Beschkov V. (1994) Biotechnol. Lett. 16, 715 – 720  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[26] Milsom P. E., Merrs J. L. (1985) Compr. Biotechnol. 3, 681 – 700.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus