The present study deals with the survey of helminth parasites from Marathwada region (M.S.) India, during July 2008 to June 2009 this report summarizes the data of incidence, intensity and density of infection of helminth parasites in freshwater fishes in relation to environmental factors. Fish samples were collected from four main localities i.e. Osmanabad, Aurangabad, Latur and Nanded in Marathwada region examined for helminth parasites included three classes i.e. Cestode, Trematode and Nematode. During the present study 879 fishes were examined, in which 487 fishes were infected with seven genera of helminth parasites among these four were cestodes, two were trematodes and one was nematode. The present studies are helpful for the status of diversity of helminth parasites from Marathwada region