COMBINED EFFECT OF GA3 AND GAMMA RADIATION ON SEED GERMINATION OF RHEUM EMODI WALL

RUCHI SINGH¹*, SURENDRA KUMAR², G.C. JOSHI³, PREETI CHATURVEDI^4
1Department of Biological Sciences, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, 263145, Uttarakhand
²Radiations & Isotopic Tracers Laboratory, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, 263145, India
³Radiations & Isotopic Tracers Laboratory, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, 263145, India
⁴Department of Biological Sciences, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, 263145, Uttarakhand
* Corresponding Author : ruchisingh12apr@gmail.com

Received : 02-12-2018     Accepted : 12-12-2018     Published : 15-12-2018
Volume : 10     Issue : 23       Pages : 7605 - 7607
Int J Agr Sci 10.23 (2018):7605-7607

Keywords : R. emodi, gamma radiation, doses, seed germination, GA3
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to University Grant Commission (UGC) for providing the research fellowship. Authors are also thankful to College of Basic Sciences and Humanities, G. B. Pant University of Agriculture & Technology, Pantnagar, 263145, Uttarakhand, India
Author Contribution : All authors equally contributed

Present study was aimed to determine the combined effect of gamma radiation and GA3 on seed germination of Rheum emodi. The seeds were pre-soaked in five different conc. of GA3 for 48 h before irradiation with five different dosages (20.0 -100.0 Gy) of gamma radiation. Seeds were subsequently placed in Petri dishes with Whatman filter paper and moistened with dist. Water. Seeds treated with GA3 (400.0 ppm) without irradiation showed maximum seed germination percentage (96.66%) and seed vigour index (3779.167). Germination index was also maximum (35.89) in seeds treated with GA3 (500.0 ppm) without irradiation and minimum in the irradiated(100 Gy) seeds with GA3(100 ppm). Gamma rays alone and in combination with GA3 showed inhibitory effect on various parameters of seed germination even at the lowest dosage (20 Gy).Contrary to gamma radiation, GA3 exhibited stimulatory effect on seed germination.

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