MOLECULAR VARIABILITY OF Pyricularia grisea ISOLATES FROM PRAKASAM AND SRI POTTI SREERAMULU NELLORE DISTRICT OF ANDHRA PRADESH

VARAPRASADA RAO¹*, P. ANIL KUMAR^2
1ICAR-Krishi Vigyan Kendra, Darsi, Prakasam, 523247, Acharya N. G. Ranga Agricultural University, Lam, Guntur, 522034, Andhra Pradesh, India
²Department of Plant Pathology, Agricultural College, Bapatla, 522101, Acharya N. G. Ranga Agricultural University, Lam, Guntur, 522034, Andhra Pradesh, India
* Corresponding Author : varaprasadakvk@gmail.com

Received : 13-05-2018     Accepted : 19-05-2018     Published : 30-05-2018
Volume : 10     Issue : 10       Pages : 6010 - 6014
Int J Agr Sci 10.10 (2018):6010-6014

Keywords : Pyricularia grisea, Random Amplified Polymorphic DNA (RAPD) analysis, Dendrogram, Similarity Degree
Academic Editor : Dr J. R. Talaviya
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Agricultural College, Bapatla, 522101, Acharya N. G. Ranga Agricultural University, Lam, Guntur, 522034, Andhra Pradesh, India.
Author Contribution : All author equally contributed

The molecular variability of twelve isolates of Pyricularia grisea Sacc. causing blast of rice was studied by using Random Amplified Polymorphic DNA (RAPD) analysis. The twelve isolates were divided into two major clusters A and B based on their Similarity Degree (SD) value higher than 0.25. The cluster A is divided into A1 and A2. The sub group A2 comprised PKM 3 and sub group A1 divided into A3 and A4 comprised NLR 6 and PKM 5 isolates. The cluster B was further divided into groups B1 and B2. The cluster B2 comprised PKM 2 isolate, the B1 group was further divided into sub groups B3 and B4. The sub group B4 comprised PKM 6 and sub group B3 was divided into B 5 and B6. The B5 included PKM 1 and PKM 4 and NLR 1, NLR 5, NLR 2, NLR 3 and NLR 4 were grouped in B6.

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