IN-VITRO MORPHOGENESIS STUDIES IN Gerbera jamesonii BOLUS EX HOOKER F

DEEPA BHATT¹, M.K. TRIPATHI², M. VIDHYA SANKAR³, R.P. PATEL⁴, R. JATAV⁵*
¹Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur, 458001, Madhya Pradesh, India
²Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur, 458001, Madhya Pradesh, India
³Department of Floriculture and Landscaping, KNK-College of Horticulture, Mandsaur, 458001, Madhya Pradesh, India
⁴Department of Plant Pathology, KNK-College of Horticulture, Mandsaur, 458001, Madhya Pradesh, India
⁵Departmentof Horticulture, College of Agriculture, Gwalior, 474002, Madhya Pradesh, India
* Corresponding Author : rajehort02@rediffmail.com

Received : 11-08-2016     Accepted : 07-09-2016     Published : 30-10-2016
Volume : 8     Issue : 52       Pages : 2542 - 2547
Int J Agr Sci 8.52 (2016):2542-2547

Keywords : Gerbera jamesonii, Mature embryo culture, Direct and indirect organogenesis, Direct and indirect somatic embryogenesis, Embryogenesis and plantlet regeneration
Academic Editor : Nagaraja A.
Conflict of Interest : None declared
Acknowledgements/Funding : None declared
Author Contribution : None declared

During present investigation, mature embryos were used as explant sources and cultured on MS medium amended with diverse auxins and cytokinins in varying concentrations as sole as well as in different combinations Culture media MS3D.5B (MS + 3.0 mg. l-1 2, 4 D + 0.5 mgl-1BA + 30.0 gl-1 sucrose + 7.5 gl-1 agar powder) proved well for callus initiation. Culture media MS2N.5iP/MS3N.5ip (MS + 2.0/3.0mg l-1 NAA + 0.5 mgl-12-ip + 30.0 gl-1 sucrose + 7.5 gl-1 agar) exhibited higher response i.e. number of shoot proliferating explants and number of shoot (s) per explant. However, shoot of higher length was recorded on nutrient medium MSB/MS2B (MS + 2.0/3.0 mgl-1 BA + 30.0 gl-1 sucrose + 7.5 g l-1 agar). Higher rooting response (root proliferating efficiency, number of roots and mean root length) was exhibited by rooting medium MS.1IB (MS + 0.1 mgl-1IBA + 15.0gl-1 sucrose + 7.5 g l-1 agar). The plantlets were transferred to pots and hardened in Environmental Growth Cabinet and Net House during initial weaning period and transferred to field successfully. Phenotypic normal plants were recovered.