COMPARATIVE PARASITOLOGICAL AND IMMUNOLOGICAL RESPONSE OF RESISTANT GAROLE AND SUSCEPTIBLE SAHABADI SHEEP TO EXPERIMENTALLY INDUCED HAEMONCHOSIS

R. JAS¹*, G. BORDOLOI², S. DAS³, A. BRAHMA⁴, D. KUMAR⁵, S. PANDIT⁶, S. BAIDYAAND⁷, J.D. GHOSH^8
1Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700 037, India
²Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700 037, India
³Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India
⁴Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India
⁵Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India
⁶Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India
⁷Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India
⁸Department of Veterinary Parasitology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India
* Corresponding Author : rumajas@gmail.com

Received : 08-07-2016     Accepted : 28-07-2016     Published : 07-08-2016
Volume : 8     Issue : 3       Pages : 187 - 190
Int J Parasitol Res 8.3 (2016):187-190

Keywords : Garole sheep, Haemonchus contortus, Immunological response, Resistance, Resilience
Academic Editor : Dr Ajit Kumar
Conflict of Interest : None declared
Acknowledgements/Funding : The authors thankfully acknowledge the financial assistance of the Indian Council of Agricultural Research, New Delhi in conducting this study under the research project entitled “All India Network Programme on Gastrointestinal Parasitism”.
Author Contribution : None declared

Resistance / resilience of Garole sheep to induced Haemonchus contortus infection was explored by comparing the parasitological and immunological indicators with the known susceptible Sahabadi breed of sheep. Ten animals from both the breeds were orally infected with third stage infective larvae (L3) of Haemonchus contortus @ 700 L3 per kg body weight and six animals of each breed were maintained as respective controls. Pre-patent period of the infection and faecal egg count of all the infected sheep was recorded at 3 days interval from 18 days post infection (DPI) to 42 DPI. No significant difference in pre-patent period was observed. Garole sheep had significantly (P< 0.05) lower faecal egg count (FEC) than the Sahabadi sheep up to 24 DPI and after that it was negative. Detection and titration of H. contortus specific IgG was performed by indirect enzyme linked immune sorbent assay (ELISA) and the parasite specific serum IgG response as well as titre of IgG was significantly (P< 0.05) higher in Garole sheep compared to Sahabadi sheep. The results of the present study indicated that Garole sheep was comparatively more resistant and / or resilient to H. contortus than Sahabadi sheep.