PHYSIOLOGICAL AND BIOCHEMICAL EVALUATION OF BACKCROSS INBRED LINES IN RICE FOR SALINITY AND SUBMERGENCE TOLERANCE

D. DHIVYAPRIYA¹*, A. KALAMANI², M. RAVEENDRAN³, S. ROBIN^4
1Department of Rice, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003
²Department of Forage Crops, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003
³Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003
⁴Department of Rice, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003
* Corresponding Author : 89ddp06@gmail.com

Received : 15-03-2016     Accepted : 21-03-2016     Published : 21-05-2016
Volume : 8     Issue : 17       Pages : 1303 - 1307
Int J Agr Sci 8.17 (2016):1303-1307

Keywords : BILs, Biochemical and Physiological Evaluation, Rice, Salinity and Submergence
Academic Editor : Dr S Ramchander, R. S. Badere
Conflict of Interest : None declared
Acknowledgements/Funding : None declared
Author Contribution : None declared

Salinity and submergence tolerant lines in Improved White Ponni genetic background were developed by Marker Assisted Backcrossing by using FL478 and FR13A as the donors. Salinity and submergence tolerant BILs viz., IWP-10-13-3-20-2-2, IWP-10-13-3-20-2-5, IWP-10-13-3-20-2-7 and IWP-10-13-3-56-2-7 were developed by inter-crossing the independent lines to pyramid SalT and Sub1 QTLs in IWP background. The BILs were evaluated for salinity and submergence tolerance independently along with their parents. Biochemical and physiological evaluation were done to select the best salinity/submergence tolerant BILs. Ion concentration (Na+/K+), H2O2 level and antioxidant enzyme activity were determined under salt stress condition (100 mM NaCl). Total sugars and chlorophyll content were determined before and after submergence. Underwater shoot elongation, survival per cent and yield were also recorded during submergence screening. Generally, BILs showed enhanced tolerance to salinity/submergence compared to IWP. Among the BILs, IWP-10-13-3-20-2-2 had salinity tolerant traits such as increased level of antioxidant enzymes, decreased level of H2O2, very low Na+/K+ ratio under salt stress. Similarly, under submergence screening, IWP-10-13-3-20-2-2 had high amount of total sugars and chlorophyll content after submergence. Also IWP-10-13-3-20-2-2 had comparatively lesser underwater shoot elongation and produced high yield. Hence IWP-10-13-3-20-2-2 performed well in both salinity and submergence screening and it can be used as a parent to develop multiple stress tolerance variety by pyramiding QTLs for other abiotic stress.