STANDARDIZATION OF MEDIA COMBINATIONS FOR HARDENING OF ORCHID DENDROBIUM Spp Cv. SONIA-17

S. MUNA¹*, S.K. BEURA², M. BISWAL³, B. TARIA⁴, P. SAHU^5
1Department of Floriculture and Landscaping, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Orissa
²Department of Floriculture and Landscaping, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Orissa
³Department of Floriculture and Landscaping, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Orissa
⁴Department of Floriculture and Landscaping, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Orissa
⁵Department of Vegetable Science, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Orissa
* Corresponding Author : monalisha.horti@gmail.com

Received : 02-02-2016     Accepted : 10-02-2016     Published : 28-02-2016
Volume : 8     Issue : 6       Pages : 1042 - 1044
Int J Agr Sci 8.6 (2016):1042-1044

Keywords : Dendrobium spp, Sonia 17, Coco peat, Coconut husk, Charcoal, Ex-agar plantlet
Academic Editor : Asha, Saghfi Siamak
Conflict of Interest : None declared
Acknowledgements/Funding : None declared
Author Contribution : None declared

The present investigation was carried out on “standardization of media mixture for hardening of in vitro plantlets with an objective to develop protocol for hardening of the In-vitro grown Dendrobium cv. Sonia 17 with 8 different treatment combinations. Viz. T1-cocopeat; T2-cocopeat +1pc coconut husk; T3-cocopeat +2pc coconut husk; T4-cocopeat +3pc coconut husk; T5-cocopeat + charcoal (1: 1); T6-cocopeat + charcoal + 1pc coconut husk; T7-cocopeat + charcoal + 2pc coconut husk; T8-cocopeat + charcoal + 3pc coconut husk. Treatments consisting of plantlets 3 pieces coconut husk tied with the ex-agar plantlets and planted in the pro-tray containing coco peat (T4) significantly enhanced the formation of new leaf/plantlet (5.3)no of roots/plantlets(7.5), length of the root (2.5 cm) and percentage of survival (96.67%) of the plantlets. While lowest values in terms of new leaf/plantlet (4.17 )no of roots/plantlets(6.33 ), length of the root (1.83 cm) and percentage of survival (53.33%) are obtained from treatment consisting of coco peat media only.