Xba I POLYMORPHISM OF ESTROGEN RECEPTOR ALPHA (ESR1) GENE AND ITS ASSOCIATION WITH TYPE 2 DIABETIC POST-MENOPAUSAL WOMEN OF GUJARAT

PATEL A.¹, BAVSHAR K.², DABHI B.³, LAL S.⁴, MISTRY K.^5
1Ashok and Rita Patel Inst. of Integrated Study & Res. in Biotechnology and Allied Sciences, New Vallabh Vidyanagar- 388 121, Gujarat, India.
²Ashok and Rita Patel Inst. of Integrated Study & Res. in Biotechnology and Allied Sciences, New Vallabh Vidyanagar- 388 121, Gujarat, India.
³Ashok and Rita Patel Inst. of Integrated Study & Res. in Biotechnology and Allied Sciences, New Vallabh Vidyanagar- 388 121, Gujarat, India.
⁴Ashok and Rita Patel Inst. of Integrated Study & Res. in Biotechnology and Allied Sciences, New Vallabh Vidyanagar- 388 121, Gujarat, India.
⁵Ashok and Rita Patel Inst. of Integrated Study & Res. in Biotechnology and Allied Sciences, New Vallabh Vidyanagar- 388 121, Gujarat, India.

Received : 28-11-2013     Accepted : 18-12-2013     Published : 28-12-2013
Volume : 2     Issue : 1       Pages : 21 - 24
World Res J Diabetes 2.1 (2013):21-24

Diabetes mellitus type 2 is a systemic disease characterized by hyperglycemic status, which is chiefly caused by absence of insulin or insulin insensitivity. Menopause women who experience estrogen associated type 2 diabetes mellitus shown to increase rapidly. Xba I polymorphism in ESR1 gene may decrease the expression of estrogen receptor activity there by increasing the risk of developing type 2 diabetes in post menopausal women. In this study we aimed to evaluate the Xba I polymorphism in ESR1 gene and its association with type 2 diabetes mellitus in post menopausal women of Gujarat. 84 menopause women were recruited for the study consisted 42 women with T2DM and 42 women as control (without T2DM). Xba I polymorphism were determined by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). The absence of the Xba I restriction site were indicated by “X1” and presence were indicated by “X2”. Chi square test were used in statistical analysis to measure hardy Weinberg equilibrium (HWE) and risk of X1/X2 allele for suffering T2DM. Xba I genotype was distributed as 21% (X1X1), 50% (X1X2), and 29% (X2X2) in diabetic women. X1 allele frequency is 46.4% while X2 allele frequency is 53.5% in diabetic women. Logistic regression analysis of genotype distribution of Xba I in both diabetic and normal post menopausal women revealed that there was no significant association of Xba I ESR1 gene polymorphism and type 2 diabetes.