The plant leaves contain a number of medicinally considerable compounds. The present investigation was carried out to explore the phytochemicals present in the <i>Moringa oleifera</i> plant leaves to evaluate and compare the antimicrobial activity of <i>Moringa oleifera</i> leaves against <i>Aeromonas eucrenophila, Enterobacter aerogenes, Morganella morganii, E. coli, Bacillus casei, Cunninghamella bertholletiae, Aspergillusnidulans, Aspergillus flavus, Apergillus niger, Mucor hemalis.</i> Antimicrobial activity of 50, 100, 150 and 100% ethyl acetate extract of <i>Moringa oleifera</i> has been evaluated against above pathogens. In 100% and 150% concentration, ethyl acetate extract of <i>Moringa oleifera</i> showed the highest 23mm and 25mm antimicrobial zone respectively against <i>Morganella morganii</i>. While, 19mm, 20mm antimicrobial zone respectively against <i>Apergillus flavus</i>. In 150% concentration, it has highest 18mm, 19mm, 20mm and 21mm antimicrobial zone respectively against <i>Enterobacter aerogenes, Apergillusnidulans, Bacillus casei, Aeromonas eucrenophila.</i> The leaves of ethyl acetate extract of <i>Moringa oleifera</i> contain alkaloids, steroids, terpenoids, phenols, saponin, tannin, carbohydrate. The extract showed inhibitory effects due to the presence of above phytoconsistuents.