CONTROLLING OF Pseudomonas cichorii AND Dickeya dadantii (Erwinia chrysanthemi) BY ELECTROSPUN NANOFIBERS OF NYLON-6/CHITOSAN BLENDS

ABDEL-MEGEED A.¹*, SADIK M.W.², EIFAN S.A.³, EL-NEWEHY M.H.^4
1Department of Plant Protection, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt.
²Department of Microbiology, Faculty of Agriculture, Cairo University, Giza 12613.
³Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box: 2455 Riyadh 11451, Saudi Arabia.
⁴Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
* Corresponding Author : aamahmoud@ksu.edu.sa

Received : 20-08-2013     Accepted : 23-09-2013     Published : 30-12-2013
Volume : 5     Issue : 1       Pages : 155 - 162
Int J Biotechnol Appl 5.1 (2013):155-162
DOI : http://dx.doi.org/10.9735/0975-2943.5.1.155-162

Keywords : Chitosan, nylon-6, glycidyltrimethyl ammonium chloride, Pseudomonas cichorii, Dickeya dadantii (Erwinia chrysanthemi)
Conflict of Interest : None declared
Acknowledgements/Funding : The authors extend their appreciation to the Deanship of Scientific Research at King Saud University, for funding the work through the research group project No RGP-VPP-010

This is the first report to use electrospun nanofibers which could be of considerable interest to the development of new antibacterial compounds against certain species of bacteria affect plants in different ways as bacterial leaf spot (bacterial midrib rot), Pseudomonas cichorii and bacterial blight Dickeya dadantii (Erwinia chrysanthemi). Electrospun nylon-6/chitosan (nylon-6/Ch) nanofibers were obtained from formic acid as a single solvent. Surface modification of electrospun nylon-6/chitosan nanofibers was observed by soaking the mat in aqueous solution of glycidyltrimethylammonium chloride (GTMAC) at room temperature overnight to give nylon-6/N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan chloride (nylon-6/HTCC). The morphological, structural and thermal properties of the nylon-6/chitosan nanofibers were studied by field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, and thermogravimetric analysis (TGA). Biological screening demonstrated that Nylon-6/HTCC mat exhibited high potential antibacterial activity against Pseudomonas cichorii and Dickeya dadantii (Erwinia chrysanthemi) on protein profile. Pseudomonas cichorii and Dickeya dadantii (Erwinia chrysanthemi) examined using SEM were totally deformed and exhibited severe destruction.

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