ASSAY OF NEW FORMULATIONS OF ISOSORBIDE MONONITRATE BY USING UV SPECTROPHOTOMETER

DILSHAD H.¹, SAFILA N.², NAQVI S.B.^3
1Department of Pharmaceutical Sciences, Faculty of Pharmacy, Karachi University Jinnah University for Women, Karachi-74600, Pakistan.
²Department of Pharmaceutical Sciences, Faculty of Pharmacy, Karachi University Jinnah University for Women, Karachi-74600, Pakistan.
³Department of Pharmaceutical Sciences, Faculty of Pharmacy, Karachi University Jinnah University for Women, Karachi-74600, Pakistan.

Received : 07-11-2013     Accepted : 12-12-2013     Published : 21-12-2013
Volume : 1     Issue : 1       Pages : 9 - 10
World Res J Med 1.1 (2013):9-10

A rapid, simple, accurate, and economical spectrophotometric method has been developed and validated for the assay of isosorbide mononitrate in new tablet formulation and compare with different brand available in the market. The analysis is based on the UV absorbance maxima at about 405nm wavelength of isosorbide mononitrate, using glacial acetic acid as solvent. A sample of drug was dissolved in glacial acetic acid to produce a solution containing isosorbide mononitrate. Similarly, a sample of ground tablets of different brand were extracted with glacial acetic acid and diluted with the same glacial acetic acid. The absorbance of sample preparation was measured at 405 nm against the solvent blank and the assay was determined by comparing with the absorbance of a similarly prepared standard solution of isosorbide mononitrate. The method can be applied for the routine QC quantitation of isosorbide mononitrate in tablet formulation.