IMMUNOPROTEOMICS APPROACH FOR DEVELOPMENT OF MHC BINDERS AND FRAGMENT BASED PEPTIDE VACCINES FROM TETRAHYMENA PYRIFORMIS

Gomase V.S.¹, Kapoor R.A.², Ladak S.S.^3
1School of Technology, S.R.T.M. University, Sub-Centre, Latur, 413512, India
²Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
³Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India

Received : -     Accepted : -     Published : 15-06-2010
Volume : 1     Issue : 1       Pages : 7 - 11
J Infect Dis Lett 1.1 (2010):7-11

Tetrahymena pyriformis are free-living ciliate protozoa that Guppies are easily susceptible to Tetrahymena. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in parasitic diseases. Analysis shows MHC class II binding peptides of antigen protein from Tetrahymena pyriformis are important determinant for protection of host form parasitic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 389 amino acids, which shows 381 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Tetrahymena pyriformis.

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