IMMUNO-PROTEOMICS APPROACH FOR SYNTHETIC VACCINE DEVELOPMENT FORM HAEMOPHILUS INFLUENZAE

Gomase V.S.¹, Kapoor R.A.², Ladak S.S.^3
1School of Technology, S.R.T.M. University, Sub-Centre, Latur, 413512, India
²Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
³Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India

Received : -     Accepted : -     Published : 15-06-2010
Volume : 1     Issue : 1       Pages : 1 - 6
J Infect Dis Lett 1.1 (2010):1-6

Haemophilus influenzae causes systemic infections leading to be an obligatory step in infection. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen protein from Haemophilus influenzae are important determinant for protection of host form pathogenic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 575 amino acids, which shows 567 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Haemophilus influenzae.

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