ISOLATION, PURIFICATION & CHARACTERIZATION OF GLUCOSE ISOMERASE ENZYME FORM STREPTOMYCES SPECIES ISOLATED FROM PARBHANI REGION

Prashant Srivastava¹, Saurabh Shukla², Sanjay Kumar Choubey³, Gomase V.S.^4
1Yeshwant College of Information Technology (Bioinformatics & Biotechnology), Parbhani, MS
²Yeshwant College of Information Technology (Bioinformatics & Biotechnology), Parbhani, MS
³Yeshwant College of Information Technology (Bioinformatics & Biotechnology), Parbhani, MS
⁴School of Technology, S.R.T.M. University, Sub-Centre, Latur, 413512, India

Received : -     Accepted : -     Published : 15-06-2010
Volume : 1     Issue : 1       Pages : 1 - 10
J Enzym Res 1.1 (2010):1-10

Bacteria are the rich sources of various enzymes. Streptomyces spices are mostly used for production of various enzymes, antibiotics etc. There product may be extracellular or intracellular. The Glucose Isomerase (GI) is the third most commercially important enzyme. We have carried out screening and isolation of glucose isomerase (GI) producing bacteria from corn field of the Parbhani region. Different isolates was found to be grown on salt starch agar medium at 35 of which only sreptomyces like colony. Characteristics showing colony was taken and found to be producer of intracellular glucose isomerase on synthetic medium at pH 6.5 containing xylose and along with salt solutions. The optimum conditions for production were 1.5% initial xylose concentration, 1% peptone, 2% Inoculum, pH 6.5 and temperature 35 at shaken culture condition. The glucose isomerase was isolated by cell disruption, centrifugation and then it was purified by ammonium sulphate precipitation. The enzyme activity was assayed. The results shown that sreptomyces species was good producers of glucose isomerase.

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