DETECTION OF EXTENDED SPECTRUM BETA LACTAMASES IN BLOOD CULTURE ISOLATES

Rajesh K.R.¹*, Mathavi S.², Indra Priyadarsini R.^3
1Department of Microbiology, Vinayaka Mission’s Kirupananda Variyar Medical College, Salem, 636308,Tamilnadu, India
²Department of Microbiology, Vinayaka Mission’s Kirupananda Variyar Medical College, Salem, 636308,Tamilnadu, India
³Department of Microbiology, Vinayaka Mission’s Kirupananda Variyar Medical College, Salem, 636308,Tamilnadu, India
* Corresponding Author : biocare.rajesh@gmail.com

Received : -     Accepted : -     Published : 21-12-2010
Volume : 1     Issue : 2       Pages : 1 - 3
J Clin Res Lett 1.2 (2010):1-3
DOI : http://dx.doi.org/10.9735/0976-7061.1.2.1-3

Keywords : ESBL, Blood culture, Gram negative bacilli
Conflict of Interest : None declared

Background and Objectives: ESBLs have evolved as an important mechanism of resistance in Gram-negative bacteria. Our study is designed to detect ESBL-producing Gram negative Bacilli from blood culture isolates. Materials and Methods: Totally 548 blood samples, collected over a period of 6months were tested. The blood samples were directly added to brain heart infusion (BHI) broth and incubated at 370C. Subculture was made into MacConkey and Blood agar plates. The susceptibility of the pure culture isolates to Third Generation Cephalosporins (3 GC)- Ceftriaxone, Ceftazidime, Cefotaxime and to the other antibiotics were tested by Disc Diffusion Method. Gram Negative Bacilli isolates with resistance or with reduced susceptibility to Ceftriaxone, Ceftazidime and Cefotaxime were selected for the study. Phenotypic confirmation of ESBLs is done by Double Disc Synergy Test. Results: A total of 548 blood samples were collected, out of which there was growth of Gram Negative Bacilli in 63 samples in which 27 isolates were found to be ESBL producers (42.85 %).Conclusion: The emergence and spread of organisms producing Extended-spectrum Beta lactamases can be prevented by repeated surveillance and prudent use of antibiotics.

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