IMMUNOPROTEOMICS APPROACH FOR DEVELOPMENT OF SYNTHETIC PEPTIDE VACCINE FROM MYCOBACTERIUM TUBERCULOSIS

Gomase V.S.¹*, Chitlange N.R.^2
1School of Technology, S.R.T.M. University, Sub-Centre, Latur, 413531, MS, India
²School of Technology, S.R.T.M. University, Sub-Centre, Latur, 413531, MS, India
* Corresponding Author : gomase.viren@gmail.com

Received : -     Accepted : -     Published : 15-06-2009
Volume : 1     Issue : 1       Pages : 7 - 12
Int J Immunol Res 1.1 (2009):7-12

Keywords : Mycobacterium tuberculosis, antigen n, epitope, PSSM, SVM, MHC, peptide vaccine Abbreviations: Goldman, Engelberg and Steitz, (GES); major histocompatibility complex, (MHC); Position Specific Scoring Matrices, (PSSMs); Support Vector Machine, (SVM)
Conflict of Interest : None declared

Mycobacterium tuberculosis causes tuberculosis leading to be an obligatory step in infection. Peptide fragments of antigen can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in bacterial diseases. Analysis shows MHC class II binding peptides of antigen from Mycobacterium tuberculosis are important determinant for protection of host form tuberculosis infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 338 amino acids, which shows 330 nonamers. Binding ability prediction of antigen peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from Mycobacterium tuberculosis antigen.

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