C¹⁴ GLUCOSE UPTAKE DURING IN Vitro ORGANOGENESIS OF SUGARCANE

Patil C.S.¹, Gangawane A.K.², Anjali R.³, Bhagwat M.P.⁴, Naik G.R.^5
1Department of Biotechnology, B.V. Bhoomareddy College, Bidar-585 403, Karnataka, India
²Department of Biotechnology and Bioinformatics, Dr. D.Y. Patil University, Plot no 50, Sector 15, CBD, Belapur, Navi Mumbai-400614
³Department of Biotechnology and Bioinformatics, Dr. D.Y. Patil University, Plot no 50, Sector 15, CBD, Belapur, Navi Mumbai-400614
⁴Department of Biotechnology and Bioinformatics, Dr. D.Y. Patil University, Plot no 50, Sector 15, CBD, Belapur, Navi Mumbai-400614
⁵Department of Biotechnology, Gulbarga University, Gulbarga-585 106, Karnataka, India

Received : -     Accepted : -     Published : 15-06-2010
Volume : 1     Issue : 1       Pages : 6 - 8
Int J Mol Biol 1.1 (2010):6-8
DOI : http://dx.doi.org/10.9735/0976-0482.1.1.6-8

Keywords : sugarcane, callus, organogenesis, regeneration, C14 glucose uptake.
Conflict of Interest : None declared

Sugarcane tissue culture is frequently used for the developmental studies during organogenesis .Organogenesis is complicated process involving cellular, molecular and tissue level change in the metabolism. The unorganized mass of cells differentiates in to shoots by undergoing modifications in the metabolic reaction etc. It is necessary to study there metabolic change by investigating glucose utilization pattern. However there are no reports on C¹⁴ glucose uptakes studies in 3 organogenetic stages. During short term feeding the highest glucose activity is observed in callus stage and it declines as the tissue dedifferentiates into shoots. Similar pattern is observed during long term exposure. It indicates that C¹⁴ glucose utilization pattern depends on the organogenetic stage and its requirements are higher at the initial callus stage than in the completely regenerative shoots.

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