PROTECTIVE ROLE OF Î²-CAROTENE FROM CHLOROCOCCUM HUMICOLA AGAINST REACTIVE OXYGEN SPECIES AND LIPID PEROXIDATION IN B(A)P INDUCED TOXICITY

BHAGAVATHY S.; SUMATHI P.

doi:http://dx.doi.org/10.9735/0976-7134.1.2.21-35

PROTECTIVE ROLE OF Î²-CAROTENE FROM CHLOROCOCCUM HUMICOLA AGAINST REACTIVE OXYGEN SPECIES AND LIPID PEROXIDATION IN B(A)P INDUCED TOXICITY

BHAGAVATHY S.¹*, SUMATHI P.²
¹Bharathiar University, Coimbatore, Tamilnadu, , Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai-600 119, Tamilnadu, India
²Department of Biochemistry, SRM Arts and Science College, Chennai, Tamilnadu
* Corresponding Author : bhagavathy12@rediffmail.com

Received : 24-11-2010 Accepted : 20-12-2010 Published : 21-12-2010
Volume : 1 Issue : 2 Pages : 21 - 35
J Pharmacol Res 1.2 (2010):21-35
DOI : http://dx.doi.org/10.9735/0976-7134.1.2.21-35

Cite - MLA : BHAGAVATHY S. and SUMATHI P. "PROTECTIVE ROLE OF Î²-CAROTENE FROM CHLOROCOCCUM HUMICOLA AGAINST REACTIVE OXYGEN SPECIES AND LIPID PEROXIDATION IN B(A)P INDUCED TOXICITY." Journal of Pharmacology Research 1.2 (2010):21-35. http://dx.doi.org/10.9735/0976-7134.1.2.21-35

Cite - APA : BHAGAVATHY S., SUMATHI P. (2010). PROTECTIVE ROLE OF Î²-CAROTENE FROM CHLOROCOCCUM HUMICOLA AGAINST REACTIVE OXYGEN SPECIES AND LIPID PEROXIDATION IN B(A)P INDUCED TOXICITY. Journal of Pharmacology Research, 1 (2), 21-35. http://dx.doi.org/10.9735/0976-7134.1.2.21-35

Cite - Chicago : BHAGAVATHY S. and SUMATHI P. "PROTECTIVE ROLE OF Î²-CAROTENE FROM CHLOROCOCCUM HUMICOLA AGAINST REACTIVE OXYGEN SPECIES AND LIPID PEROXIDATION IN B(A)P INDUCED TOXICITY." Journal of Pharmacology Research 1, no. 2 (2010):21-35. http://dx.doi.org/10.9735/0976-7134.1.2.21-35

Copyright : © 2010, BHAGAVATHY S. and SUMATHI P., Published by Bioinfo Publications. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: The mechanism of Benzo(a)Pyrene toxicity is complicated which alters the physiological system and the production of its by products have severe adverse effects. It is important to verify the mechanism of B(a)P induced damage in order to perform cancer chemotherapy with natural substances. Dietary intake of Î²-carotene plays a beneficial role in lowering oxidative stress, highly accumulated in fresh water green algae Chlorococcum humicola. Methods: The Î²-carotene was extracted, purified from C. humicola by column chromatographic method, the structure was confirmed by UV-VIS, NMR and MASS spectrophotometric analysis. Four groups of Swiss albino mice were treated as control, B(a)P, Î²-carotene, B(a)P+Î²-carotene respectively for a period of 30 days. Production of Reactive Oxygen species (ROS) and Total antioxidant capacity (TAC) were assessed in the blood sample. Lipid peroxidation and the antioxidant status were assessed in various tissue samples. Histopathology of liver was observed. Results: The levels of ROS and lipidperoxidation increased in blood and in collected tissues respectively show the severity of the tissue damage. The TAC, enzymic antioxidants (CAT, SOD and GPx) and non enzymic antioxidants (GSH, Vit A, Vit C, and Vit E) showed significant changes compared with the control mice. The histopathology of liver also confirms the protection of Î²-carotene from the cellular damage. Conclusion: The overall findings demonstrate the animals post treated with Î²-carotene from C. humicola may prevent toxicity induced by carcinogenic chemical and hence aid in establishing the chemo preventive effect. Therefore C.humicola can be further extended to exploits its possible application for various health benefits as neutraceuticals and food additives.

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