Optimization of growth and production of protease by Penicillium species using submerged fermentation

Vamsi Krishna K¹, Mayank Gupta², Nikhil Gupta³, Hipal Gaudani⁴, Soham Trivedi⁵, Prasad Patil⁶, Girish Gupta⁷, Yogesh Khairnar⁸, Amol Borasate⁹, Dharmendra Mishra^10
1Department of Biotechnology, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
²Department of Biotechnology, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
³Department of Biotechnology, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
⁴Department of Biotechnology, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
⁵Department of Biotechnology, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
⁶Department of Biotechnology, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
⁷Department of Biotechnology, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
⁸Department of Biotechnology, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
⁹Department of Biotechnology, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
¹⁰Senior Research Coordinator, Reliable analytical laboratory, Thane

Received : -     Accepted : -     Published : 15-06-2009
Volume : 1     Issue : 1       Pages : 14 - 18
Int J Microbiol Res 1.1 (2009):14-18
DOI : http://dx.doi.org/10.9735/0975-5276.1.1.14-18

Conflict of Interest : None declared

Enzymes play a vital role in the industry with a wide range of application. Agricutural waste is the maximum waste been produced in India. This Project aims at using various agricultural wastes for the production of Protease. To achieve this various research is being conducted. Various Bacterial and Fungal species are being used for the production of Protease. Penicillium species was being used in this work for the production of Protease .In this work the various components of the media were studied and were optimized and used for the production of Protease.

[1] Hameed A.C., Natt M.A. and Evans C.S. (1996) World J. Microbiol. Biotech. 12,289-291  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[2] Ito S., Kobayashi T., Ara K., Ozaki K., Kawai S., Hatada Y. (1998) Extremophiles, 2,185-190  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[3] Mohsen Fathi Najafi, Dileep Deobagkar, Deepti Deobagkar. (2005) Electronic J. of Biotechnology, 8, 2  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[4] Ishikawa H., Ishimi K., Sugiura M., Sowa A. and Fujiwara N. (1993) Journal of Fermentation and Bioengineering, 76, 4, 300-305.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[5] Gupta R., Beg Q.K., Khan S., Chauhan B. (2002) Applied Microbiology and Biotechnology, 60,381-395.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[6] Hossain M.S., Iqbal M.S. and Hoq M.M. (2004) Bangladesh J. Microbiol, 21,86- 89  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[7] Bradford M. (1976) Analytical Biochemistry 72, 248-254  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[8] Stanbury P.F, Whitaker A., Hall J.S. Principles of Fermentation Technology, 2’nd edition, Pergamon press, 14, 167- 272  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[9] Aneja K.R. Experiments in Microbiolog, plant pathology, tissue culture and Mushroom production technology, 3’rd edition, New Age International Publishers, New Delhi, 357-358  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[10] Kannan N., Handbook of Laboratory Culture Media, Reagents, Stains and Buffer, Panima Publishing Corporation New Delhi/Bangalore, 126-127  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[11] Rao M. B., Tanksale A. M., Ghatge M .S. and Deshpande V. V. (1998) Microbiol. Mol Biol. Rev. 62, 597-635  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[12] Friedrich J., Gradisar H., Mandin D. and Chaumont J. P. (1999) Lett. Appl. Microbiol 28,127-130  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[13] Boyer P. D. (1971) The enzymes, 3rd ed. Academic Press, Inc., New York, N.Y  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[14] Hoffman T (1974) Adv. Chem. Ser. 136, 146– 185.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[15] International Union of Biochemistry. (1992) Enzyme nomenclature. Aca-demic Press, Inc., Orlando, Fl  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[16] Barett A. J. (1994) Methods Enzymol. 244, 1– 15.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[17] Menon A. S. and Goldberg A. L. (1987) J. Biol. Chem. 262, 14929– 14934.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[18] Argos P. (1987) J. Mol. Biol. 193, 385– 396.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[19] Rawlings N. D. and Barrett A. J. (1993) Biochem. J. 290, 205– 218  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[20] Cerny G. (1978) Eur. J. Appl. Microbiol. Bio-technol, 5, 113– 122.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[21] Barrett A.J., Rawlings N.D. and Wessner J. F. (eds). (1998) Handbook of proteolytic enzymes, Academic Press  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus