ISSN : 0975-4423
EISSN : 0975-914X
Gomase V.S.1*, Shankar S.2, Gangawane A.K.3, Sherkhane A.S.4
1Proteomics Unit, Department of Bioinformatics, Padmashree Dr. D. Y. Patil University, Plot No-50, Sector-15, CBD Belapur, Navi Mumbai, 400614, MS, India
2Proteomics Unit, Department of Bioinformatics, Padmashree Dr. D. Y. Patil University, Plot No-50, Sector-15, CBD Belapur, Navi Mumbai, 400614, MS, India
3Proteomics Unit, Department of Bioinformatics, Padmashree Dr. D. Y. Patil University, Plot No-50, Sector-15, CBD Belapur, Navi Mumbai, 400614, MS, India
4Department of Biotechnology, College of computer Science and information Technology, Latur, 413531, MS, India
* Corresponding Author : virusgene1@yahoo.co.in
Received : - Accepted : - Published : 15-06-2009
Volume : 1 Issue : 1 Pages : 18 - 20
Int J Drug Discov 1.1 (2009):18-20
DOI : http://dx.doi.org/10.9735/0975-4423.1.1.18-20
Keywords : Antigen, Epitope, PSSM, SVM, MHC, Peptide vaccine
Conflict of Interest : None declared
The toxin LTx5 toxin is an 116aa residue peptide is isolated from the venom of Lasiodora sp. IBSP8539.In this assay we have predicted the binding affinity of toxin LTx5 toxin having 116 amino acids, which shows 109 nonamers. Peptide fragments of the neurotoxin can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in neurotoxin studies. Small segment ‘3- STFIIMISLAVALATWPSEH-22’ of toxin protein called the antigenic epitopes is sufficient for eliciting the desired immune response. Immunization cassettes should be capable of immunizing of broad immunity against both humoral and cellular epitope thus giving vaccines the maximum ability to deal with neurotoxin protein of Eurypelma californicum. Binding ability prediction of antigen peptides to MHC class molecules is important in vaccine development, We also found the SVM based MHCII-IAb peptide regions, 40- YALADRAEK, 10- ISLAVALAT, 99- MYQAERALE, 14- VALATWPSE, (optimal score is 1.494); MHCII-IAd peptide regions, 96- LDIMYQAER, 29- SETKLNVEL, 68- GASVLCEAV, 4- STFIIMISL, (optimal score is 0.604); MHCII-IAg7 peptide regions , 38- GPYALADRA, 98- IMYQAERAL, 9- MISLAVALA, 99- MYQAERALE, (optimal score is 1.904); and MHCII- RT1.B peptide regions, 17- ATWPSEHIE, 101- QAERALEKL, 98- IMYQAERAL, 108- KLASSFRCE, (optimal score is 0.601) which represented predicted binders from neurotoxin protein. We have predicted a successful immunization.
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