Kaliwal B.B.1*, Sanakal R. D.2, Kurjogi M. M.3
1P.G. Department of Studies in Biotechnology and Microbiology, Karnatak University, Dharwad, 580003, India
2P.G. Department of Studies in Biotechnology and Microbiology, Karnatak University, Dharwad, 580003, India
3P.G. Department of Studies in Biotechnology and Microbiology, Karnatak University, Dharwad, 580003, India
* Corresponding Author : b_kaliwal@yahoo.com
Received : - Accepted : - Published : 21-12-2010
Volume : 2 Issue : 2 Pages : 33 - 40
Int J Biotechnol Appl 2.2 (2010):33-40
DOI : http://dx.doi.org/10.9735/0975-2943.2.2.33-40
Keywords : Staphylococcus aureus. Pigment. Antioxidant. CCl4 carotinoid, staphyaloxanthin
thiobarbituric acid reactive substances, glutathione Reduced glutathione
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are grateful to the Post Graduate
Department of Studies in Microbiology and
Biotechnology, Karnatak University Dharwad
for providing the necessary facilities
The present investigation was under taken to select a suitable antibiotic for the treatment of bovine mastitis by conducting antibiotic sensitivity test with different antibiotics and to evaluate the antioxidant property of pigment staphyloxanthin produced by S. aureus against Carbon tetrachloride (CCl4) induced stress in albino mice. In this study a total of 20 samples were screened and out of which 12 samples confirmed Staphylococcus aureus were obtained. The confirmed strains of Staphylococcus aureus were subjected to haemolytic activity. α, β and non-haemolytic activities were, 33.33%, 50% and 16.67% observed respectively among the isolates of Staphylococcus aureus. Antibiotic susceptibility of staphylococcus aureus against 5 antibiotics was tested by using standard antibiotic discs. Ceftriaxone was found to be the most effective drug and also mice is used as a model to study the antioxidant property of staphylococcus aureus pigment against a stress induced by CCl4 in mice. Results showed that animals treated with 1ml/kg body weight of CCl4 for 2 days caused a marked rise in the level of thiobarbituric acid reactive substances (TBARS) and decreased glutathione Reduced glutathione (GSH), Superoxide dismutase (SOD), Catalase (CAT) and Glutathione-S-transferase (GST) levels in the liver, kidney and testis tissue homogenates of CCl4 treated mice. Graded doses of Staphylococcus aureus pigment Staphyloxanthin successfully prevented the alterations of these effects in the experimental mice.
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