Evaluating antioxidant potential, cytotoxicity and intestinal absorption of flavonoids extracted from medicinal plants.

Sharma R.J.¹*, Chaphalkar S.R.², Adsool A.D.^3
1VidyaPratishthan’s, Arts, Science and Commerce College Vidyanagari, Baramati, 413 133, Pune
²VidyaPratishthan’s, Arts, Science and Commerce College Vidyanagari, Baramati, 413 133, Pune
³VidyaPratishthan’s, Arts, Science and Commerce College Vidyanagari, Baramati, 413 133, Pune
* Corresponding Author : raajesh_sharma2004@yahoo.com

Received : -     Accepted : -     Published : 15-06-2010
Volume : 2     Issue : 1       Pages : 1 - 5
Int J Biotechnol Appl 2.1 (2010):1-5
DOI : http://dx.doi.org/10.9735/0975-2943.2.1.1-5

Keywords : Flavonoids, DPPH assay, MTT assay
Conflict of Interest : None declared
Acknowledgements/Funding : The current work is funded by ‘BCUD’, University of Pune; carried out at ‘VidyaPrathisthan School of Biotechnology’, Baramati. I extend my thanks to ‘Indian Red Cross Society’ Baramati for allowing me to use Eliza reader. I am thankful to

Plants described in Ayurvedic literature namely, A. catechu, E. officinalis, F. benghalensis, F. glomerata, and T. arjuna possess significant amount of flavonoids as secondary metabolites. These compounds are reported to exhibit various biological activities, including antioxidative and free radical scavenging activities. Antioxidant compounds in food play an important role as a health-protecting factor. Scientific evidence suggests that antioxidants reduce the risk for chronic diseases including cancer and heart disease. The flavonoids extracted from bark of these plants were fractionated using HPTLC. These were assessed for the antioxidant potential employing DPPH [1, 1- Diphenyl- 2- picrylhydrazyl] assay. Simultaneously the flavonoids showing comparatively better activity were tested for their Cytotoxicity using MTT [Mitochondrial toxicity test]. The preparation was also subjected for absorption studies in goat intestinal model system.

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